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pegfp myo10 hmm bird  (Addgene inc)


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    Addgene inc pegfp myo10 hmm bird
    Pegfp Myo10 Hmm Bird, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+myo10+hmm+bird/pEGFP-MYO10-HMM+(Plasmid+%2387256)/pm41923617-556-131-135
    Average 92 stars, based on 4 article reviews
    pegfp myo10 hmm bird - by Bioz Stars, 2026-09
    92/100 stars

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    Polymerase Chain Reaction:

    Article Title: TMEM145 is a principal component of outer hair cell stereocilia.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-TMEM145 polyclonal antibody Atlas Antibodies Cat# HPA060462; RRID:AB_2684285 TUB antibody Proteintech Cat# 17928-1-AP; RRID:AB_2212236 Anti-STRC polyclonal antibody Eurogentec N/A ARL13B antibody Proteintech Cat# 17711-1-AP; RRID:AB_2060867 Anti-KCNQ4 Antibody Alomone Labs Cat# APC-164; RRID:AB_2341042 Mouse Anti-Eps8 Monoclonal Antibody, Unconjugated, Clone 15 BD Biosciences Cat# 610144; RRID:AB_397545 Recombinant Anti-LHFPL5 antibody [EPR16285] - C-terminal Abcam Cat# ab192242; RRID:AB_2942040 Anti-CDH23 Antibody Prof. Ulrich Müller, John Hopkins University55 N/A DYKDDDDK tag antibody Proteintech Cat# 80801-2-RR; RRID: AB_3670488 V5-tag antibody Proteintech Cat# 81775-4-RR; RRID:AB_3670504 HA tag antibody Proteintech Cat# 51064-2-AP; RRID:AB_11042321 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 488 Conjugated Thermo Fisher Scientific Cat# A-11008; RRID:AB_143165 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 568 Conjugated Thermo Fisher Scientific Cat# A-11011; RRID:AB_143157 Goat Anti-Mouse IgG (H+L) Highly Crossadsorbed Antibody, Alexa Fluor® 647 Conjugated Thermo Fisher Scientific Cat# A-21236; RRID:AB_2535805 IRDye® 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 StarBright® Blue 700 Goat Anti-Mouse IgG Bio-Rad Cat# 12004159; RRID:AB_2884948 FluoTag®-X2 anti-ALFA AbberiorStar635P NanoTag Biotechnologies Cat# N1502-AB635P-L; RRID:AB_3075979 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Protease inhibitor cocktail Sigma-Aldrich Cat#P8349 PMSF solution Merck Cat#10837091001 Triton® X-100 ROTH Cat#3051.3 jetPRIME® Polyplus Cat#101000046 Polyethylenimine (PEI) Custom-made N/A LipofectamineTM 2000 Transfection Reagent Thermo Fisher Scientific Cat#11668030 FM1-43 dye solution MedChemExpress Cat#HY-D1434 GSK-A1 SYNkinase Cat#SYN-1219-M001 DAPI Nucleic Acid Stain Thermo Fisher Scientific Cat#D1306 Phalloidin-iFluor 633 Reagent Abcam Cat#ab176758 Phalloidin-iFluor 405 Reagent Abcam Cat#ab176752 1,2-Bis(2-Aminophenoxy)ethaneN,N,N′,N′-tetraacetic acid (BAPTA) Sigma-Aldrich Cat#A4926 Poly-D-lysine Thermo Fisher Scientific Cat#A3890401 Critical commercial assays SuperScript IV Reverse Transcriptase Thermo Fisher Scientific Cat#18090010 (Continued on next page) ll OPEN ACCESS Article e1 Neuron 114, 1–15.e1–e7, August 5, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse brain total extracted RNA Ambion N/A Phusion High-Fidelity DNA Polymerase Thermo Fisher Scientific Cat#F530S PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 EZviewTM Red ANTI-FLAG® M2 Affinity Gel Millipore Cat#F2426 Experimental models: Cell lines HEK293, Human Thermo Fisher Scientific Cat#11625019 HeLa, Human Prof. Ralf Jakob, Marburg56 N/A LLC-PK1-CL4, Pig James R. Bartles57 N/A Experimental models: Organisms/strains C57BL/6NCrl Charles River N/A C57BL/6NCrlTmem145em1(IMPC)Mbp/Mmucd MMRRC RRID:MMRRC_065589-UCD Tmem145mandolin/mandolin Potter et al.26 N/A STRC-KO Shubina-Oleinik et al.15 N/A Oligonucleotides See Table S1 N/A Recombinant DNA mTmem145-pEGFP-N1 This paper NM_183311.2 mTmem145ΔC-pEGFP-N1 This paper NM_183311.2 mTmem145-HA-pCDNA3.1(+) Genescript NM_183311.2 mTmem145-alfatag-pCDNA3.1(-) Genescript NM_183311.2 mTmem145-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔGOLD-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔC-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mSTRC-Myc-pCDNA3.1(+) This paper NM_021885.4; Subcloning from Shubina-Oleinik et al.15 TagBFP2-CAAX This paper N/A pcDNA3.1-MYO10-HMM-Nanotrap Bird et al.25 Addgene; Cat87255 pEGFP-MYO10-HMM Bird et al.25 Addgene; Cat#87256 mTubby-DsREDmono-pEGFP-C1 Thallmair et al.42 NM_021885.4; mTubby-pRFP-C1 Thallmair et al.42 NM_021885.4; mTubby-V5-pCDNA3.1(+) Genescript NM_021885.4 Software and algorithms Graphpad Prism v5.0 GraphPad Software https://www.graphpad.com Igor Pro v8.0.4.2 Wavemetrics https://www.wavemetrics.com/ Fiji v2.14.0 Schneider et al.58 https://fiji.sc/ PYMOL v2.1.0 Schrodinger https://www.pymol.org/ Biorender Biorender https://www.biorender.com/ Adobe Illustrator v30.0 Adobe Inc. https://www.adobe.com/ Snapgene Dotmatics https://www.snapgene.com/ RStudio v4.4.0 Posit https://posit.co/ Superplot package Lord et al.59 N/A Interface Residues script SB Grid Consortium https://pymolwiki.org/InterfaceResidues NanoSPD ImageJ macro This paper DOI:10.5281/zenodo.18840150 Other AlphaFold Jumper et al.32 https://alphafoldserver.com ll OPEN ACCESSArticle Neuron 114, 1–15.e1–e7, August 5, 2026 e2 ..

    Recombinant:

    Article Title: TMEM145 is a principal component of outer hair cell stereocilia.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-TMEM145 polyclonal antibody Atlas Antibodies Cat# HPA060462; RRID:AB_2684285 TUB antibody Proteintech Cat# 17928-1-AP; RRID:AB_2212236 Anti-STRC polyclonal antibody Eurogentec N/A ARL13B antibody Proteintech Cat# 17711-1-AP; RRID:AB_2060867 Anti-KCNQ4 Antibody Alomone Labs Cat# APC-164; RRID:AB_2341042 Mouse Anti-Eps8 Monoclonal Antibody, Unconjugated, Clone 15 BD Biosciences Cat# 610144; RRID:AB_397545 Recombinant Anti-LHFPL5 antibody [EPR16285] - C-terminal Abcam Cat# ab192242; RRID:AB_2942040 Anti-CDH23 Antibody Prof. Ulrich Müller, John Hopkins University55 N/A DYKDDDDK tag antibody Proteintech Cat# 80801-2-RR; RRID: AB_3670488 V5-tag antibody Proteintech Cat# 81775-4-RR; RRID:AB_3670504 HA tag antibody Proteintech Cat# 51064-2-AP; RRID:AB_11042321 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 488 Conjugated Thermo Fisher Scientific Cat# A-11008; RRID:AB_143165 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 568 Conjugated Thermo Fisher Scientific Cat# A-11011; RRID:AB_143157 Goat Anti-Mouse IgG (H+L) Highly Crossadsorbed Antibody, Alexa Fluor® 647 Conjugated Thermo Fisher Scientific Cat# A-21236; RRID:AB_2535805 IRDye® 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 StarBright® Blue 700 Goat Anti-Mouse IgG Bio-Rad Cat# 12004159; RRID:AB_2884948 FluoTag®-X2 anti-ALFA AbberiorStar635P NanoTag Biotechnologies Cat# N1502-AB635P-L; RRID:AB_3075979 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Protease inhibitor cocktail Sigma-Aldrich Cat#P8349 PMSF solution Merck Cat#10837091001 Triton® X-100 ROTH Cat#3051.3 jetPRIME® Polyplus Cat#101000046 Polyethylenimine (PEI) Custom-made N/A LipofectamineTM 2000 Transfection Reagent Thermo Fisher Scientific Cat#11668030 FM1-43 dye solution MedChemExpress Cat#HY-D1434 GSK-A1 SYNkinase Cat#SYN-1219-M001 DAPI Nucleic Acid Stain Thermo Fisher Scientific Cat#D1306 Phalloidin-iFluor 633 Reagent Abcam Cat#ab176758 Phalloidin-iFluor 405 Reagent Abcam Cat#ab176752 1,2-Bis(2-Aminophenoxy)ethaneN,N,N′,N′-tetraacetic acid (BAPTA) Sigma-Aldrich Cat#A4926 Poly-D-lysine Thermo Fisher Scientific Cat#A3890401 Critical commercial assays SuperScript IV Reverse Transcriptase Thermo Fisher Scientific Cat#18090010 (Continued on next page) ll OPEN ACCESS Article e1 Neuron 114, 1–15.e1–e7, August 5, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse brain total extracted RNA Ambion N/A Phusion High-Fidelity DNA Polymerase Thermo Fisher Scientific Cat#F530S PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 EZviewTM Red ANTI-FLAG® M2 Affinity Gel Millipore Cat#F2426 Experimental models: Cell lines HEK293, Human Thermo Fisher Scientific Cat#11625019 HeLa, Human Prof. Ralf Jakob, Marburg56 N/A LLC-PK1-CL4, Pig James R. Bartles57 N/A Experimental models: Organisms/strains C57BL/6NCrl Charles River N/A C57BL/6NCrlTmem145em1(IMPC)Mbp/Mmucd MMRRC RRID:MMRRC_065589-UCD Tmem145mandolin/mandolin Potter et al.26 N/A STRC-KO Shubina-Oleinik et al.15 N/A Oligonucleotides See Table S1 N/A Recombinant DNA mTmem145-pEGFP-N1 This paper NM_183311.2 mTmem145ΔC-pEGFP-N1 This paper NM_183311.2 mTmem145-HA-pCDNA3.1(+) Genescript NM_183311.2 mTmem145-alfatag-pCDNA3.1(-) Genescript NM_183311.2 mTmem145-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔGOLD-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔC-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mSTRC-Myc-pCDNA3.1(+) This paper NM_021885.4; Subcloning from Shubina-Oleinik et al.15 TagBFP2-CAAX This paper N/A pcDNA3.1-MYO10-HMM-Nanotrap Bird et al.25 Addgene; Cat87255 pEGFP-MYO10-HMM Bird et al.25 Addgene; Cat#87256 mTubby-DsREDmono-pEGFP-C1 Thallmair et al.42 NM_021885.4; mTubby-pRFP-C1 Thallmair et al.42 NM_021885.4; mTubby-V5-pCDNA3.1(+) Genescript NM_021885.4 Software and algorithms Graphpad Prism v5.0 GraphPad Software https://www.graphpad.com Igor Pro v8.0.4.2 Wavemetrics https://www.wavemetrics.com/ Fiji v2.14.0 Schneider et al.58 https://fiji.sc/ PYMOL v2.1.0 Schrodinger https://www.pymol.org/ Biorender Biorender https://www.biorender.com/ Adobe Illustrator v30.0 Adobe Inc. https://www.adobe.com/ Snapgene Dotmatics https://www.snapgene.com/ RStudio v4.4.0 Posit https://posit.co/ Superplot package Lord et al.59 N/A Interface Residues script SB Grid Consortium https://pymolwiki.org/InterfaceResidues NanoSPD ImageJ macro This paper DOI:10.5281/zenodo.18840150 Other AlphaFold Jumper et al.32 https://alphafoldserver.com ll OPEN ACCESSArticle Neuron 114, 1–15.e1–e7, August 5, 2026 e2 ..

    Subcloning:

    Article Title: TMEM145 is a principal component of outer hair cell stereocilia.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-TMEM145 polyclonal antibody Atlas Antibodies Cat# HPA060462; RRID:AB_2684285 TUB antibody Proteintech Cat# 17928-1-AP; RRID:AB_2212236 Anti-STRC polyclonal antibody Eurogentec N/A ARL13B antibody Proteintech Cat# 17711-1-AP; RRID:AB_2060867 Anti-KCNQ4 Antibody Alomone Labs Cat# APC-164; RRID:AB_2341042 Mouse Anti-Eps8 Monoclonal Antibody, Unconjugated, Clone 15 BD Biosciences Cat# 610144; RRID:AB_397545 Recombinant Anti-LHFPL5 antibody [EPR16285] - C-terminal Abcam Cat# ab192242; RRID:AB_2942040 Anti-CDH23 Antibody Prof. Ulrich Müller, John Hopkins University55 N/A DYKDDDDK tag antibody Proteintech Cat# 80801-2-RR; RRID: AB_3670488 V5-tag antibody Proteintech Cat# 81775-4-RR; RRID:AB_3670504 HA tag antibody Proteintech Cat# 51064-2-AP; RRID:AB_11042321 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 488 Conjugated Thermo Fisher Scientific Cat# A-11008; RRID:AB_143165 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 568 Conjugated Thermo Fisher Scientific Cat# A-11011; RRID:AB_143157 Goat Anti-Mouse IgG (H+L) Highly Crossadsorbed Antibody, Alexa Fluor® 647 Conjugated Thermo Fisher Scientific Cat# A-21236; RRID:AB_2535805 IRDye® 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 StarBright® Blue 700 Goat Anti-Mouse IgG Bio-Rad Cat# 12004159; RRID:AB_2884948 FluoTag®-X2 anti-ALFA AbberiorStar635P NanoTag Biotechnologies Cat# N1502-AB635P-L; RRID:AB_3075979 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Protease inhibitor cocktail Sigma-Aldrich Cat#P8349 PMSF solution Merck Cat#10837091001 Triton® X-100 ROTH Cat#3051.3 jetPRIME® Polyplus Cat#101000046 Polyethylenimine (PEI) Custom-made N/A LipofectamineTM 2000 Transfection Reagent Thermo Fisher Scientific Cat#11668030 FM1-43 dye solution MedChemExpress Cat#HY-D1434 GSK-A1 SYNkinase Cat#SYN-1219-M001 DAPI Nucleic Acid Stain Thermo Fisher Scientific Cat#D1306 Phalloidin-iFluor 633 Reagent Abcam Cat#ab176758 Phalloidin-iFluor 405 Reagent Abcam Cat#ab176752 1,2-Bis(2-Aminophenoxy)ethaneN,N,N′,N′-tetraacetic acid (BAPTA) Sigma-Aldrich Cat#A4926 Poly-D-lysine Thermo Fisher Scientific Cat#A3890401 Critical commercial assays SuperScript IV Reverse Transcriptase Thermo Fisher Scientific Cat#18090010 (Continued on next page) ll OPEN ACCESS Article e1 Neuron 114, 1–15.e1–e7, August 5, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse brain total extracted RNA Ambion N/A Phusion High-Fidelity DNA Polymerase Thermo Fisher Scientific Cat#F530S PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 EZviewTM Red ANTI-FLAG® M2 Affinity Gel Millipore Cat#F2426 Experimental models: Cell lines HEK293, Human Thermo Fisher Scientific Cat#11625019 HeLa, Human Prof. Ralf Jakob, Marburg56 N/A LLC-PK1-CL4, Pig James R. Bartles57 N/A Experimental models: Organisms/strains C57BL/6NCrl Charles River N/A C57BL/6NCrlTmem145em1(IMPC)Mbp/Mmucd MMRRC RRID:MMRRC_065589-UCD Tmem145mandolin/mandolin Potter et al.26 N/A STRC-KO Shubina-Oleinik et al.15 N/A Oligonucleotides See Table S1 N/A Recombinant DNA mTmem145-pEGFP-N1 This paper NM_183311.2 mTmem145ΔC-pEGFP-N1 This paper NM_183311.2 mTmem145-HA-pCDNA3.1(+) Genescript NM_183311.2 mTmem145-alfatag-pCDNA3.1(-) Genescript NM_183311.2 mTmem145-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔGOLD-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔC-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mSTRC-Myc-pCDNA3.1(+) This paper NM_021885.4; Subcloning from Shubina-Oleinik et al.15 TagBFP2-CAAX This paper N/A pcDNA3.1-MYO10-HMM-Nanotrap Bird et al.25 Addgene; Cat87255 pEGFP-MYO10-HMM Bird et al.25 Addgene; Cat#87256 mTubby-DsREDmono-pEGFP-C1 Thallmair et al.42 NM_021885.4; mTubby-pRFP-C1 Thallmair et al.42 NM_021885.4; mTubby-V5-pCDNA3.1(+) Genescript NM_021885.4 Software and algorithms Graphpad Prism v5.0 GraphPad Software https://www.graphpad.com Igor Pro v8.0.4.2 Wavemetrics https://www.wavemetrics.com/ Fiji v2.14.0 Schneider et al.58 https://fiji.sc/ PYMOL v2.1.0 Schrodinger https://www.pymol.org/ Biorender Biorender https://www.biorender.com/ Adobe Illustrator v30.0 Adobe Inc. https://www.adobe.com/ Snapgene Dotmatics https://www.snapgene.com/ RStudio v4.4.0 Posit https://posit.co/ Superplot package Lord et al.59 N/A Interface Residues script SB Grid Consortium https://pymolwiki.org/InterfaceResidues NanoSPD ImageJ macro This paper DOI:10.5281/zenodo.18840150 Other AlphaFold Jumper et al.32 https://alphafoldserver.com ll OPEN ACCESSArticle Neuron 114, 1–15.e1–e7, August 5, 2026 e2 ..

    Software:

    Article Title: TMEM145 is a principal component of outer hair cell stereocilia.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-TMEM145 polyclonal antibody Atlas Antibodies Cat# HPA060462; RRID:AB_2684285 TUB antibody Proteintech Cat# 17928-1-AP; RRID:AB_2212236 Anti-STRC polyclonal antibody Eurogentec N/A ARL13B antibody Proteintech Cat# 17711-1-AP; RRID:AB_2060867 Anti-KCNQ4 Antibody Alomone Labs Cat# APC-164; RRID:AB_2341042 Mouse Anti-Eps8 Monoclonal Antibody, Unconjugated, Clone 15 BD Biosciences Cat# 610144; RRID:AB_397545 Recombinant Anti-LHFPL5 antibody [EPR16285] - C-terminal Abcam Cat# ab192242; RRID:AB_2942040 Anti-CDH23 Antibody Prof. Ulrich Müller, John Hopkins University55 N/A DYKDDDDK tag antibody Proteintech Cat# 80801-2-RR; RRID: AB_3670488 V5-tag antibody Proteintech Cat# 81775-4-RR; RRID:AB_3670504 HA tag antibody Proteintech Cat# 51064-2-AP; RRID:AB_11042321 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 488 Conjugated Thermo Fisher Scientific Cat# A-11008; RRID:AB_143165 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 568 Conjugated Thermo Fisher Scientific Cat# A-11011; RRID:AB_143157 Goat Anti-Mouse IgG (H+L) Highly Crossadsorbed Antibody, Alexa Fluor® 647 Conjugated Thermo Fisher Scientific Cat# A-21236; RRID:AB_2535805 IRDye® 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 StarBright® Blue 700 Goat Anti-Mouse IgG Bio-Rad Cat# 12004159; RRID:AB_2884948 FluoTag®-X2 anti-ALFA AbberiorStar635P NanoTag Biotechnologies Cat# N1502-AB635P-L; RRID:AB_3075979 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Protease inhibitor cocktail Sigma-Aldrich Cat#P8349 PMSF solution Merck Cat#10837091001 Triton® X-100 ROTH Cat#3051.3 jetPRIME® Polyplus Cat#101000046 Polyethylenimine (PEI) Custom-made N/A LipofectamineTM 2000 Transfection Reagent Thermo Fisher Scientific Cat#11668030 FM1-43 dye solution MedChemExpress Cat#HY-D1434 GSK-A1 SYNkinase Cat#SYN-1219-M001 DAPI Nucleic Acid Stain Thermo Fisher Scientific Cat#D1306 Phalloidin-iFluor 633 Reagent Abcam Cat#ab176758 Phalloidin-iFluor 405 Reagent Abcam Cat#ab176752 1,2-Bis(2-Aminophenoxy)ethaneN,N,N′,N′-tetraacetic acid (BAPTA) Sigma-Aldrich Cat#A4926 Poly-D-lysine Thermo Fisher Scientific Cat#A3890401 Critical commercial assays SuperScript IV Reverse Transcriptase Thermo Fisher Scientific Cat#18090010 (Continued on next page) ll OPEN ACCESS Article e1 Neuron 114, 1–15.e1–e7, August 5, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse brain total extracted RNA Ambion N/A Phusion High-Fidelity DNA Polymerase Thermo Fisher Scientific Cat#F530S PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 EZviewTM Red ANTI-FLAG® M2 Affinity Gel Millipore Cat#F2426 Experimental models: Cell lines HEK293, Human Thermo Fisher Scientific Cat#11625019 HeLa, Human Prof. Ralf Jakob, Marburg56 N/A LLC-PK1-CL4, Pig James R. Bartles57 N/A Experimental models: Organisms/strains C57BL/6NCrl Charles River N/A C57BL/6NCrlTmem145em1(IMPC)Mbp/Mmucd MMRRC RRID:MMRRC_065589-UCD Tmem145mandolin/mandolin Potter et al.26 N/A STRC-KO Shubina-Oleinik et al.15 N/A Oligonucleotides See Table S1 N/A Recombinant DNA mTmem145-pEGFP-N1 This paper NM_183311.2 mTmem145ΔC-pEGFP-N1 This paper NM_183311.2 mTmem145-HA-pCDNA3.1(+) Genescript NM_183311.2 mTmem145-alfatag-pCDNA3.1(-) Genescript NM_183311.2 mTmem145-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔGOLD-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔC-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mSTRC-Myc-pCDNA3.1(+) This paper NM_021885.4; Subcloning from Shubina-Oleinik et al.15 TagBFP2-CAAX This paper N/A pcDNA3.1-MYO10-HMM-Nanotrap Bird et al.25 Addgene; Cat87255 pEGFP-MYO10-HMM Bird et al.25 Addgene; Cat#87256 mTubby-DsREDmono-pEGFP-C1 Thallmair et al.42 NM_021885.4; mTubby-pRFP-C1 Thallmair et al.42 NM_021885.4; mTubby-V5-pCDNA3.1(+) Genescript NM_021885.4 Software and algorithms Graphpad Prism v5.0 GraphPad Software https://www.graphpad.com Igor Pro v8.0.4.2 Wavemetrics https://www.wavemetrics.com/ Fiji v2.14.0 Schneider et al.58 https://fiji.sc/ PYMOL v2.1.0 Schrodinger https://www.pymol.org/ Biorender Biorender https://www.biorender.com/ Adobe Illustrator v30.0 Adobe Inc. https://www.adobe.com/ Snapgene Dotmatics https://www.snapgene.com/ RStudio v4.4.0 Posit https://posit.co/ Superplot package Lord et al.59 N/A Interface Residues script SB Grid Consortium https://pymolwiki.org/InterfaceResidues NanoSPD ImageJ macro This paper DOI:10.5281/zenodo.18840150 Other AlphaFold Jumper et al.32 https://alphafoldserver.com ll OPEN ACCESSArticle Neuron 114, 1–15.e1–e7, August 5, 2026 e2 ..



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    pegfp myo10 hmm bird  (Addgene inc)
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    Addgene inc pegfp myo10 hmm bird
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    https://www.bioz.com/product/pegfp+myo10+hmm+bird/pEGFP-MYO10-HMM+(Plasmid+%2387256)/pm41923617-556-131-135
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